AN ASSAY METHOD FOR PANTOTHENIC ACID

An assay for pantothenic acid in biologic fluids.

ANTOTHENIC ACID DEFICIENCY reduces biologic actylations and leads to a variety of pathologic changes in blood cholesterol partition and antibody production. Deficiency affects particularly the adrenal cortex, nervous system, the skin, and hair; it also affects longevity (1, 2, 3, 4). The ubiquity of pantothenic acid (as suggested by its name), makes it difficult to demonstrate deficiencies, eve...

The pantothenic acid content of coenzyme A by chick assay.

Lipmann, Kaplan, Novelli, Tuttle, and Guirard (1) reported that, whereas concentrates of the coenzyme required for acetylation (coenzyme A) showed no pantothenic acid activity by the ordinary microbiological assay, considerable amounts of p-alanine were found after acid hydrolysis. From this early observation, it was suspected that the coenzyme might contain combined pantothenic acid which was ...

Assay and distribution of bound forms of pantothenic acid.

The distribution of CoA (I), pantetheine (2, 3), and pantothenic acid (4) among a variety of natural materials is well established. However, until now, the relative amounts of these compounds present in any one substance has not been determined. Also, data on the distribution of P-pantothenic1 acid and P-pantetheine, both of which are intermediates in the biosynthesis of CoA (5), is rather meag...

An improved method for the determination of pantothenic acid in tissues.

During work on coenzyme A (CoA), a method for the liberation of pantothenic acid from the coenzyme was worked out (1). The method involves the use of a combination of two enzymes; namely, intestinal phosphatase and an enzyme extractable from acetone powder of bird liver (chicken (2), duck, or pigeon). This procedure proved the most efficient pretreatment to liberate bound vitamin under conditio...

Microbic Decomposition of Pantothenic Acid.